Journal: Journal of Clinical Investigation

Article Title: Neonatal Fc receptor antagonist efgartigimod safely and sustainably reduces IgGs in humans

doi: 10.1172/jci97911

Figure Lengend Snippet: Figure 3. Effect of efgartigimod and IVIg on tracer IgG levels in cynomol- gus monkeys. Animals (n = 2/group) were injected with 1 mg/kg of the tracer Ab FR70-hIgG1, followed 2 days later with i.v. infusion of 70 mg/ kg efgartigimod or 2 g/kg IVIg. Tracer levels in serum, detected by murine CD70-binding ELISA, were plotted relative to predose levels. Values are shown as mean ± SEM. %T0, percentage change from baseline.

Article Snippet: Tracer Ab levels in blood serum were determined by murine CD70-binding ELISA using MaxiSorp plates (Thermo Fisher Scientific, catalog 31000) and murine CD70 (R&D Systems, catalog 783-CL/CF).

Techniques: Injection, Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Clinical Investigation

Article Title: Neonatal Fc receptor antagonist efgartigimod safely and sustainably reduces IgGs in humans

doi: 10.1172/jci97911

Figure Lengend Snippet: Figure 4. Efgartigimod dose-escalation study in cynomolgus monkeys. Animals (n = 2/group) were injected with 1 mg/kg of the tracer Ab FR70- hIgG1, followed 2 days later with i.v. infusion of 0.2 to 200 mg/kg efgar- tigimod or vehicle. Tracer levels in serum (A) are detected by murine CD70- binding ELISA. Endogenous IgG levels in serum (B) were plotted relative to predose levels. Cyno, cynomolgus. Values are shown as mean ± SEM.

Article Snippet: Tracer Ab levels in blood serum were determined by murine CD70-binding ELISA using MaxiSorp plates (Thermo Fisher Scientific, catalog 31000) and murine CD70 (R&D Systems, catalog 783-CL/CF).

Techniques: Injection, Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Distinct immune landscapes between murine and human periodontitis

doi: 10.1016/j.isci.2025.114073

Figure Lengend Snippet: Murine B/Plasma cells are fewer in number and predominantly in an early, inactive state (A) UMAP plot (left) and proportion bar chart (right) of B/plasma cell types identified in the integrated gingival dataset of mice and humans. Refer to Methods for statistical tests used. Human Δ(PD-Ctrl) vs. Murine Δ(PD-Ctrl), ∗∗ p < 0.01. (Abbreviations: mB, memory B cells; Breg, regulatory B cells). (B) UMAP plot (left) and bar chart (right) of murine and human groups in the integrated B/plasma dataset. (C) Trajectory plot of cellular pseudotime from Monocle analysis. (D) Trajectory plot of B/plasma subsets in pseudotime. (E) Trajectory plot of murine and human groups in pseudotime. (F) Immunofluorescence slices of CD138 (plasma cells), IgD (naive B cells), and CD27 (conventional memory B cells) in murine and human groups. Scale bars: 25 μm (left) and 5 μm (right). Color labels are shown in the figure. (Abbreviations: E, epithelium; L, lamina propria). (G) Combined plots show cell density changes of murine and human groups (above), B/plasma subsets (median), and gene expression changes (below) aligned with pseudotime. (H) Violin plot shows the expression level of transcription factors BTG1 and XBP1 in human groups. Data are presented as the median with interquartile range. (I) Violin plot shows the expression level of transcription factor Btg1 and Xbp1 in murine groups. Data are presented as the median with interquartile range.

Article Snippet: CD27 Recombinant Rabbit Monoclonal Antibody , Huabio , Cat #ET7107-73; RRID:AB_3070782.

Techniques: Clinical Proteomics, Immunofluorescence, Gene Expression, Expressing